| 摘要 |
The invention relates to a biosensor comprising a support (1) (prism of glass), a plasmon-carrying layer (2) that consists of a metal layer with free electrons, especially gold or silver, or a semiconductor layer and a hydrogel layer (6) that consists of a polymer, especially a dextran to which receptors (7) can be bound. Said biosensor is further provided with a protective layer (4) that consists of a silicon oxide or a metal oxide, or a protective layer (9) that consists of a polymer and that is physisorbed to the plasmon-carrying layer (2). The hydrogel layer (6) can be bound via a linker (5, 10), for example in the case of a protective layer (4) from silicon oxide by for example silane as the linker (5). The protective layer (4) has a thickness of less than 10 nm, preferably less than 5 nm, and effectively protects the active plasmon-carrying layer (2) from any disturbing contaminations. The physical optical effect produced by the surface plasmon resonance does not take place outside the range that can be measured so that the suitability as a biosensor is not lost. |
