| 摘要 |
The invention refers to the microbiological biotechnology, in particular to the processes for obtaining of the Haematococcus pluvialis green alga biomass, utilized as source of carotenoids. Summary of the invention consists in that the proposed process includes cultivation of the green alga Haematococcus pluvialis during 7 days on a nutrient medium, containing in mg:NaNO3 299...301KH2PO4 19,9...20,1K2HPO4 79,9...80,1NaCl 19,9...20,1CaCl2 46,9...47,1MgSO4 ò 7H2O 9,9...10,1ZnSO4 ò 7H2O 0,099...0,11MnSO4 ò H2O 1,49...1,51CuSO4 ò 5H2O 0,079...0,081H3BO3 0,29...0,31(NH4)6Mo7O24 ò 4H2O 0,29...0,31FeCl3 ò 6H2O 16,9...17,1Co(NO3)2 ò H2O 0,19...0,21EDTA 7,4...7,6distilled water up to 1 L. The illumination intensity constitutes 1500 lx, pH - 6,8...7,2 and the temperature - 25...27 degree C.The inoculum is added into the medium in a quantity of 0,3 g/L. The next day after cultivation in this medium is added the organic tetranorditerpenic compound 9-episclareolid in the amount of 0,025...0,075 g/L.The result of the invention consists in increasing the productivity of the alga biomass and the carotenoid synthesis.Claims: 1 |
