| 摘要 |
<p>The invention provides reagents and fluorescent metal complexes derived therefrom, for labelling target biological materials. The reagents are compounds having the following general formula: in which X1 and X2 are the same or different and are selected from hydrogen and the group: Q and Q' (if present) are the same or different and are selected from -N=CHR7- and -N=N-;Y1, Y2 (and Y3 if present) are independently selected from the group consisting of -OH, -NH2, -SH and -COOR8 where R8 is selected from H and C1-C4 alkyl;Z1, Z2 and Z3 independently represent the atoms necessary to complete one ring, two fused ring, or three fused ring aromatic or heteroaromatic systems;at least one of groups R1 to R4 (and R5, R6 and R7 if present) is the group -E-F where E is a spacer group and F is a target bonding group; andany remaining groups R1 to R4 (and R5, R6 and R7 if present) are independently selected from the group consisting of: hydrogen, halogen, amide, hydroxyl, cyano, nitro, optionally substituted amino, sulphydryl, sulphonate, sulphate, phosphonate, phosphate, quaternary ammonium, carboxyl, carbonyl, C1-C6 alkoxy, acrylate, vinyl, styryl, aryl, heteroaryl, C1-C6 alkyl, and aralkyl groups.</p> |
