NOVEL G PROTEIN-COUPLED RECEPTOR

摘要

It is intended to provide a novel G protein-coupled receptor (GPCR) gene which makes it possible to find a signal transduction mechanism in vivo or to identify a novel drug-target protein and a method of totally searching for a GPCR protein on data base. Open reading frames (ORFs) consisting of from 250 to 1000 amino acid residues are extracted from human-origin genome data and ORFs originating in DNA repeated sequences, ORFs containing many indefinite amino acids and ORFs having a single amino acid at a ratio of 20% or more are excluded therefrom. Then ORFs containing 6 to 8 transmembrane domains are extracted by using SOSUI. Among the ORFs thus obtained, a gene homologous with a known GPCR gene (preferably a gene showing the highest homology with a GPCR gene or a GPCR-associated gene in homology searching) is searched for.