| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide multifunctional embryonic stem cells, to provide a method for establishing the stem cells, and to provide a subculture method for the embryonic stem cells. SOLUTION: The objective mammalian embryonic stem cells are characterized by having all of the following cell biological characteristics: (1) derived from blastodermic embryos, (2) continuing the a proliferation in an undifferentiated condition, (3) expressing a sugar chain SSEA-1 antigen, (4) alkali phosphatase activity is positive, (5) expressing transcription factor Oct 3/4, and (6) having multidifferentiation potency. The method for establishing the embryonic stem cells comprises culturing cells obtained from the inner cell mass of mammalian blastodermic embryos on a feeder of umbilical cord endothelial cells isolated from an umbilical cord by the use of fetal bovine serum-containing MEMαas the medium to form a colony. The subculture method for the embryonic stem cells is also provided.</p> |
