| 摘要 |
In accordance with the present invention there are provided methods for producing enantiomerically pure alpha -sustituted carboxylic acids, such as, for example, alpha -amino acids and alpha -hydroxy acids, said method comprising combining an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity which stereoselectively hydrolyzes the amino nitrile or cynohydrin intermediate under conditions sufficient to produce the carboxylic acid. |
