| 摘要 |
<p>A fluorometric analyzer for analyzing the behavior of fluorescent molecules, characterized in that the analyzer comprises a laser light source, a sample stage for supporting a substrate on which a sample containing fluorescent molecules is placed, an optical system for focusing the light beam from the laser light source onto the sample to form a confocal region, a scanning mechanism for scanning the confocal region of the sample, an optical system for focusing the fluorescence from the sample, a photodetector for measuring the intensity of the focused fluorescence, and a fluorescence intensity recording means for recording the variation of the intensity of the fluorescence measured by the photodetector, the confocal region of the sample on the substrate is scanned by the scanning mechanism, the confocal region is defined near the region where the intensity of the fluorescence recorded by the fluorescence intensity recording means is maximum, the fluorescent molecules in the sample are irradiated with a laser beam after the sample is irradiated with the light beam for a predetermined time, the intensity of the fluorescence from the excited fluorescent molecules is measured, and analysis data on the fluorescent molecules is thus collected.</p> |
