摘要 |
There is provided a process of inducing luminescent emission from a luciferase bioluminescent reaction particularly useful in binding assays. A luciferin/luciferase bioluminescent combination, together with an inactiv e, caged trigger compound such as a cofactor, is subjected to photonic radiatio n, so as to release the trigger compound in active form, and thereby cause substantially instantaneous reaction of the active trigger compound so released with the luciferin/luciferase combination, to induce photonic emission which can be detected and measured.
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