CHONDROITIN LYASE ENZYMES

摘要

The present invention describes a method for the production of two highly purified enzymes capable of degrading chondroitin sulfate polysaccharides. A multi-step purification method incorporating cell disruption, cation exchang e chromatography, affinity chromatography, hydroxylapatite chromatography, hig h resolution ion exchange chromatography and size exclusion is outlined. A 77,000 ~ 5,000 Dalton protein capable of degrading chondroitin sulfates A an d C and a 55,000 ~ 2,300 Dalton protein capable of degrading dermatan sulfate were isolated. The genes encoding these enzymes, chondroitinase AC and chondroitinase B, respectively, have been cloned and methods for their use a re described.