| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for assaying a substance designed to mitigate the influence of the other components coexisting therewith in a specimen, thus useful in clinical diagnoses, and to provide a reagent for the above method. SOLUTION: This method for assaying a substance is characterized by comprising the following practice: the substance in a specimen is brought into a stoichiometric quantity of hydrogen peroxide by making use of an enzymatic reaction, the hydrogen peroxide is then reacted with a chromogen in the presence of a peroxidase and also a compound of the general formula (I) [R1 and R2 are each H or a (substituted) lower alkyl; R3 is H or joined with R1 to form NH-(CH2)n as R3-R1 (wherein, n is an integer of 2-4); R4 is a single bond or alkylene; R5 is O- or R6-X-(R6 is an optionally substituted alkylene; X is COO, SO2O or OPO2O); Y is R7-NH (R7 is an optionally 1-3 hydroxy-substituted cholanoyl group)], and a colorimetric determination is made by measuring the absorption in the visible region of the resulting colored reaction liquid by the use of an automatic analyzer.
|
