| 摘要 |
<p>A method for chromatographic preparation of tocotrienols from a liquid raw material having high contents of both tocopherols and tocotrienols, which comprises providing a chromatographic separation apparatus of pseudo mobile layer type having a number of columns being packed with a hydrophobic absorbent for reversed phase liquid chromatography having a less affinity for tocotrienols than that for tocopherols and being connected in an end-free and circulative way, feeding a substantially perfectly oily raw material containing a few tens percents of tocopherols and tocotrienols and an eluent having a composition of ethanol/water = 90/10 to the chromatographic separation apparatus, and separating a resultant liquid fraction enriched in tocotrienols from tocopherols. The method allows the chromatographic preparation of tocotrienols on a commercial scale with operations similar to those used in a conventional chromatographic separation method of pseudo mobile layer type, which leads to the reduction of the initial equipment cost and operation cost for the preparation of tocotrienols.</p> |
