| 摘要 |
<p>PCT No. PCT/EP95/00823 Sec. 371 Date Sep. 6, 1996 Sec. 102(e) Date Sep. 6, 1996 PCT Filed Mar. 6, 1995 PCT Pub. No. WO95/24503 PCT Pub. Date Sep. 14, 1995An assay for diagnosing the presence or absence of certain malignancies and their grade and prognosis which comprises a) performing in situ hybridization of a tissue of the patient to be examined with a probe derived from the H19 gene and b) evaluating the results diagnosing the presence or absence of such malignancy and its severity. The probe used is derived from the H19 gene by a) subcloning at a suitable site in a plasmid, b) producing antisense RNA by transcription with a polymerase and c) labeling suitable fragments of the H19 RNA, such that after the hybridization, a signal for the diagnosis and establishment of the severity is produced. Suitable labels are radioactive or fluorescent labels, or color change reagent. Kits are provided for effecting such assays.</p> |
