发明名称 |
METHOD AND MEDIUM FOR DETECTING/IDENTIFYING MICRO-ORGANISMS WITH ESTERASE AND/OR OSIDASE AND/OR PEPTIDASE AND/OR SULPHATASE AND/OR PHOSPHATASE ACTIVITY |
摘要 |
The invention concerns a medium for biological analysis by biochemical process comprising chromogenic and fluorogenic substrates reactive to enzymes specific (esterases and/or osidases and/or peptidases and/or sulphatases and/or phosphatases) of the searched strains. The invention aims at improving the sensitivity, initial translucency, stability and ease of use of such identification/detection media. This is achieved by the inventive medium which is in ready-to-use stable, liquid or gel form comprising at least an emulsifying stabilising agent such as ester of Sorbitan or of fatty acids/fatty acids and at least a solvent (dimethylsulphoxide). Said medium is obtained by mixing a stock solution of esterase and/or osidase and/or peptidase and/or sulphatase and/or phosphatase substrates, in the solvent and the emulsifying stabilising agent, with part of the constituents of the culture medium. The invention also concerns the preparation of such a medium and the use of ester of Sorbitan and of fatty acids/fatty acids as emulsifying stabiliser in a micro-organism detection/identification medium, in particular bacteria or yeasts, with esterase and/or osidase and/or peptidase and/or sulphatase and/or phosphatase activity. |
申请公布号 |
WO0240706(A2) |
申请公布日期 |
2002.05.23 |
申请号 |
WO2001FR03611 |
申请日期 |
2001.11.16 |
申请人 |
BIO MERIEUX;GILBERT, SANDRA;ROGER-DALBERT, CELINE;ORENGA, SYLVAIN |
发明人 |
GILBERT, SANDRA;ROGER-DALBERT, CELINE;ORENGA, SYLVAIN |
分类号 |
C12N1/00;C12N1/20;C12Q1/04;C12Q1/10;C12Q1/26;C12Q1/34;C12Q1/37;C12Q1/42;C12Q1/44;C12R1/01 |
主分类号 |
C12N1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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