发明名称 |
TEST SYSTEM FOR DETERMINING THE ACTIVITY OF CYCLO-NUCLEOTIDE-DEPENDENT PROTEIN KINASES AND VASP PHOSPHATASES |
摘要 |
The invention relates to an HTS-appropriate test system for detecting the activity of cyclo-nucleotide-dependent protein kinases (cNPK) containing: a) at least one test compound; b) at least one appropriate cNPK substrate; c) at least one composition, which is to be incubated and which contains cNPK and ATP, optionally, phosphorylation reaction stoppers, and; d) an appropriate detection system for quantifying the phosphorylation of the cNPK substrate. The invention also relates to an HTS-appropriate test system for detecting the activity of VASP phosphatases containing: e) at least one test compound; f) at least one appropriate VASP phosphatase substrate; g) at least one composition, which is to be incubated and which contains VASP phosphatase, and; h) an appropriate detection system for quantifying the dephosphorylation of the VASP phosphatase substrate. The described test systems can be used for locating compounds, which modulate the activity of a cNPK or of a VASP phosphatase, from chemical or natural substance libraries. |
申请公布号 |
WO0196594(A3) |
申请公布日期 |
2002.05.16 |
申请号 |
WO2001EP06621 |
申请日期 |
2001.06.12 |
申请人 |
VASOPHARM BIOTECH GMBH;DRUECKES, PETER;JARCHAU, THOMAS;WALTER, ULRICH;BADER, BENJAMIN |
发明人 |
DRUECKES, PETER;JARCHAU, THOMAS;WALTER, ULRICH;BADER, BENJAMIN |
分类号 |
C12Q1/48 |
主分类号 |
C12Q1/48 |
代理机构 |
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