发明名称 |
Cascade genetic circuit useful for regulated expression of cloned genes, comprises several nucleic acid constructs encoding regulators arranged in order and final target promoter responsive in dose-dependent fashion |
摘要 |
A cascade genetic circuit (I), comprising nucleic acid constructs (II) encoding several transcriptional regulators arranged in a hierarchical order so that expression of an upstream regulator stimulates expression of a downstream regulator, and a final target promoter (III) responsive in a dose-dependent fashion to a terminal downstream regulator, is new. Independent claims are also included for the following: (1) a cell comprising (I); and (2) regulating the expression of a nucleic acid, comprising: (a) establishing a genetic circuit according to the novel method; (b) placing the sequence under the control of (III); and (c) inducing the cascade genetic circuit to stimulate expression of the sequence.
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申请公布号 |
ES2167161(A1) |
申请公布日期 |
2002.05.01 |
申请号 |
ES19990001383 |
申请日期 |
1999.06.22 |
申请人 |
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS |
发明人 |
CEBOLLA RAMIREZ ANGEL;SOUSA MARTIN CAROLINA;DE LORENDO PRIETO VICTOR |
分类号 |
C12N15/67;C12N15/70;C12N15/90;(IPC1-7):C12N15/67 |
主分类号 |
C12N15/67 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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