发明名称 |
A URINE TEST FOR THE DIAGNOSIS OF PRION DISEASES |
摘要 |
The present invention relates to a method for detecting the presence of the abnormal isoform of prion protein (PrPSC) in a urine sample of a subject. The method of the invention comprising the steps of: (a) providing a urine sample of said subject; (b) isolating from said sample all proteins, preferably, isolating proteins having a molecular weight higher than about 8 Kda; (c) optionally, and preferably, subjecting the proteins obtained in step (b) to protease digestion, and isolating from the mixture obtained in step (c) any protease resistant proteins; and (d) detecting the presence of PrPSC in the protease resistant fraction obtained in step (c) by a suitable detection technique.Furthermore, the invention further relates to methods for diagnosing a prion disease in a subject and for screening donors of blood samples for the presence of prion diseases. The invention further provides for a diagnostic kit for diagnosing a prion disease in a subject.
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申请公布号 |
CA2426126(A1) |
申请公布日期 |
2002.04.25 |
申请号 |
CA20012426126 |
申请日期 |
2001.10.21 |
申请人 |
HADASIT MEDICAL RESEARCH SERVICES AND DEVELOPMENT LTD. |
发明人 |
SHAKED, GIDEON M.;GABIZON, RUTH |
分类号 |
C12Q1/37;G01N27/447;G01N33/493;G01N33/577;G01N33/68;(IPC1-7):G01N33/68 |
主分类号 |
C12Q1/37 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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