Methods of screening affinity marker libraries

摘要

A method for screening the affinity of a compound for a target molecule is disclosed, comprising the steps of immobilising the target molecule on a first substrate; incubating the compound with the target molecule under conditions that support covalent interaction between the compound and the target molecule; quenching the interaction between said target molecule and compound; and assaying for activity of the target molecule; wherein the compound is of the formula E-Ca-R-Cb-A, wherein E is a therapeutic or diagnostic agent, R is a reactive group, Cb and Ca are optional first and second connecting groups respectively, and A is an affinity group comprising any molecule or part of an molecule possessing specific binding determinants for the target molecule.