发明名称 |
BZIP TRANSCRIPTION FACTOR THAT CONTROLS EXPRESSION OF THE STORAGE PROTEIN IN THE RICE PLANT |
摘要 |
cDNAs (RISBZ1, RISBZ4 and RISBZ5) encoding bZIP type transcriptional factors are isolated from a rice seed-origin cDNA library. These cDNAs encode novel proteins and show binding activity to the GCN4 motif. RISBZ1 activates the transcription 100-fold or higher via the GCN4 motif. The expression of RISBZ 1 precedes the expression of a seed storage protein gene and observed exclusively in seeds in the ripening stage, which suggests that RISBZ1 regulates the expression of the rice seed storage protein. To facilitate the binding to the RISBZ1 transcription factor, the recognition sequence (GGN4 motif) of the transcription factor is ligated in series and transferred into a promoter of a specific storage protein. Thus, the expression of a foreign ge ne under the regulation of the thus modified promoter can be elevated.
|
申请公布号 |
CA2394018(A1) |
申请公布日期 |
2002.04.18 |
申请号 |
CA20012394018 |
申请日期 |
2001.10.11 |
申请人 |
BIO-ORIENTED TECHNOLOGY RESEARCH ADVANCEMENT INSTITUTION;NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES |
发明人 |
TAKAIWA, FUMIO;ONODERA, YASUYUKI |
分类号 |
A01H5/00;C07K14/415;C07K16/16;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/22;C12N15/09;C12N15/29;C12N15/82;C12P21/02;C12P21/08;C12Q1/02;C12R1/91;(IPC1-7):C12N15/29;C12N5/14 |
主分类号 |
A01H5/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|