FEEDBACK-RESISTANT PYRUVATE CARBOXYLASE GENE FROM CORYNEBACTERIUM

摘要

The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium . Th e mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate c arboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.