| 摘要 |
Disclosed are methods for determining DNA nucleotide sequences comprising reacting ribonucleoside 5'-triphosphates and 3'-dNTP derivatives in the presence of an RNA polymerase modified so as to enhance its ability for incorporating the 3'-dNTP derivatives and a DNA fragment containing a promoter sequence for the RNA polymerase to obtain a nucleic acid transcription product, separating the resulting nucleic acid transcription product, and determining a nucleic acid sequence from the resulting separated fraction. These methods can produce a transcription product of a long chain and afford more accurate sequence data where fluctuation of signals from labeled deoxyribonucleotides is reduced.
|
