| 摘要 |
Compositions and methods for inhibiting the cellular mismatch repair system in a plant host cell are provided. Compositions include the cDNA and amino acid sequence of a rice MLH1 ortholog. The nucleic acid molecules and proteins of the invention find use in increasing the efficiency of targeted gene mutatio n and homologous recombination in plants via inhibition of the plant cellular mismatch repair system. The plant cellular mismatch repair system is inhibit ed through the use of transposon tagging of a MLH1 gene, sense- and antisense- suppression of a MLH1 gene, antibody binding to a MLH1 polypeptide or varian t polypeptide, targeted mutagenesis of specific amino acid residues of a plant MLH1 gene, and competition with a mismatch repair impaired MLH1 polypeptide through transgeneic over-expression of the impaired polypeptide. Also provid ed are transformed plant cells, plant tissues, plants, and seeds. Additional methods that are provided include the detection of as little as one base pai r mismatch in a DNA duplex and the generation of plants with reversible male sterility for applications in hybrid generation.
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