| 摘要 |
<p>A method of analyzing a nucleic acid involving the step of extracting the subject nucleic acid to be analyzed, the amplification step and the detection step, wherein at least one internal standard nucleic acid distinguishable from the subject nucleic acid to be analyzed is added to the sample before performing the operation of each step and then the operation of each step is performed to thereby judge the success or failure of each step based on the detection result of each internal standard nucleic acid in the nucleic acid detection step and, furthermore, the analysis is carried out again based on the result of the judgement. The internal standard nucleic acids added respectively in the above steps are distinguishable from each other. This method makes it possible to judge the success or failure of the operation of each of the extraction, amplification and detection steps constituting the nucleic acid analysis. Thus, the reliability of the obtained analytical data can be improved and re-examination can be automated. This method contributes particularly to elevation of the efficiency and improvement of reliability in examination and diagnosis of gene polymorphism in the field of medicine.</p> |
