Method of identifying agents that alter the activity of the promoter sequence for corticotropin releasing-factor receptor CRF2a

摘要

A method for identifying agents that alter the activity of the promoter region of CRF2alpha receptor is disclosed. In one embodiment, the method comprises the steps of (a) obtaining a cell line or organism, wherein the cell line or organism comprises a nucleic acid sequence encoding a promoter sequence of a heterologous CRF2alpha receptor operably attached to a reporter gene and (b) introducing a test agent into the cell or transgenic animal and evaluating the expression of the reporter gene product compared to a control cell line or transgenic animal wherein the agent has not been introduced into the cell line or transgenic animal.