| 摘要 |
<p>Described are methods and compositions for generating short (cDNA) sequence tags derived from the extreme 5' ends of eukaryotic mRNAs ('5' SSTs'). The 5' SSTs may be aligned with genomic DNA sequences to elucidate the borders of the genes with their corresponding promoters. Thus, the subject invention provides for identification of genes and promoters in genomic DNA sequence and for isolation of nucleic acid molecules encoding same. Vectors comprising such nucleic acid molecules are also provided as are methods of using such nucleic acid molecules, diagnostically, therapeutically and in industrial processes. Storage medium is provided having promoter and gene sequence information in computer readable form stored thereon. In addition, the inventeion provides novel reagents and methods for conducting mRNA expression analysis. The invention also provides reagents and methods for correlating genetic polymorphisms with phenotypic traits of interest.</p> |
