Chitinase and method for preparing the same

摘要

Chitinase with the following physico-chemical properties 1 to 6:1. action: random cutting of the beta-1,4 bond of chitin to generate the tetramer and dimer of Nacetylglucosamine;2. optimum pH: 6.5 to 10.4;3. stable pH: 7.0 to 9.0;4. optimum temperature: 37° C.;5. active temperature range: 4 to 60° C.; and6. thermal stability: 60% or more of the initial activity as retained even after heating at 40° C. and pH 8.0 for 30 minutes; and a method for producing chitinase, comprising culturing a chitinase-generating bacterium reacting with chitin to generate the N-acetylglucosamine oligomer and collecting chitinase from the culture.