发明名称 |
PHOSPHATE-TRANSPORTER GENE OF NICOTIANA TABACUM L |
摘要 |
PURPOSE: A phosphate-transporter gene of Nicotiana tabacum L is provided, thereby improving the use and absorption of phosphate in plants. CONSTITUTION: The phosphate-transporter gene of Nicotiana tabacum L encodes a protein, phosphate-transporter, capable of transporting phosphate into a plant cell. The effects of phosphate-transporter gene of Nicotiana tabacum L is inspected by the steps of: preparing a primer for RT-PCR and amplifying mRNA to prepare cDNA of a potato; radioactive labeling the cDNA of a potato to isolate Ntpt1-1; subcloning Ntpt1-1 into pGEMT-Easy vector; sequencing the nucleotide sequence and amino acid sequence of Ntpt1-1; inserting NtPT1-1 into an expression vector p520 to prepare pYNtPT1-1; transforming yeast NS219 with pYNtPT1-1 and p520; and measuring the absorbency of the transformed NS219.
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申请公布号 |
KR20020013207(A) |
申请公布日期 |
2002.02.20 |
申请号 |
KR20000046695 |
申请日期 |
2000.08.11 |
申请人 |
YUN, SONG JOONG |
发明人 |
BAEK, SO HYEON;YUN, SONG JOONG |
分类号 |
C12N15/29;(IPC1-7):C12N15/29 |
主分类号 |
C12N15/29 |
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