METHOD OF PREPARING ANTIBODY BY GENE IMMUNIZATION

摘要

A method of preparing an antibody characterized by inoculating an animal with an expression vector capable of expressing a fused protein having an antigen protein fused in the C terminal side of a transmembrane domain the N terminal side of which is located in a cell while the C terminal side of which is located outside the cell, and isolating and purifying an antibody against this antigen protein from the animal. According to the method, an antibody against an antigen protein which can be hardly obtained by the conventional gene immunization methods can be prepared. The antibody thus obtained is useful in drugs, diagnostics and laboratory reagents.