发明名称 |
Synthesis of polynucleotides using combined oxidation/deprotection chemistry |
摘要 |
<p>A method of synthesizing a polynucleotide which can, for example, be used during fabrication of an array. A second nucleoside is coupled to a first nucleoside through a phosphite linkage, with the second nucleoside having a hydroxyl protecting group that is a non-carbonate protecting group. The product of the foregoing step is exposed to a composition which both oxidizes the formed phosphite to a phosphate and deprotects the protected hydroxyl of the coupled nucleoside. The method has particular application to fabricating an addressable array of polynucleotides on a substrate which carries substrate bound moieties each with a hydroxyl group. <IMAGE></p> |
申请公布号 |
EP1176151(A1) |
申请公布日期 |
2002.01.30 |
申请号 |
EP20010118360 |
申请日期 |
2001.07.27 |
申请人 |
AGILENT TECHNOLOGIES, INC. (A DELAWARE CORPORATION) |
发明人 |
DELLINGER, DOUGLAS J.;PERBOST, MICHEL G. M.;BETLEY, JASON R.;CARUTHERS, MARVIN |
分类号 |
C07H21/00;(IPC1-7):C07H21/00 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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