发明名称 |
NON-INVASIVE PRENATAL DIAGNOSIS USING CD45<-ve> FETAL CELLS |
摘要 |
CD45<-ve> fetal cells without a maternal equivalent can be purified and expanded from maternal blood in the first trimester, thereby providing abundant fetal material for genetic testing by a non-invasive route. Advantageously, the cells are adherent and CD14<-ve>, CD34<-ve>, CD68<-ve>, vWF<-ve>, myoglobin<-ve>, collagen-I<-ve>, cytokeratin<-ve>, CD44<+ve>, CD105<+ve>, CD106<+ve>, prolyl-4-hydroxylase<+ve>, fibronectin<+ve>, alpha -smooth muscle actin<+ve>, and laminin<+ve>. A process for preparing cells is disclosed, comprising the steps of (a) extracting a blood sample from a pregnant female, and (b) enriching nucleated CD45<-ve> fetal cells therein. A process for prenatal testing of a fetus for a genetic disorder comprises the further step of (c) testing the enriched nucleated CD45<-ve> fetal cell. |
申请公布号 |
WO0179851(A8) |
申请公布日期 |
2002.01.24 |
申请号 |
WO2001GB01676 |
申请日期 |
2001.04.12 |
申请人 |
IMPERIAL COLLEGE INNOVATIONS LIMITED;FISK, NICHOLAS, MAXWELL;CAMPAGNOLI, CESARE;BENNETT, PHILLIP, ROBERT;ROBERTS, IRENE, ANNE, GRAHAM |
发明人 |
FISK, NICHOLAS, MAXWELL;CAMPAGNOLI, CESARE;BENNETT, PHILLIP, ROBERT;ROBERTS, IRENE, ANNE, GRAHAM |
分类号 |
C12N5/00;C12N5/0775;G01N33/569;(IPC1-7):G01N33/569 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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