发明名称 |
SINGLE PRIMER PCR AMPLIFICATION OF RNA |
摘要 |
The invention provides methods and compositions for amplifying RNA sequences by: a) hybridizing to a target RNA a first primer comprising a 3' target RNA hybridizing sequence and a first 5' defined amplifyable sequence; b) extendi ng the first primer with a reverse transcriptase to form a first cDNA strand; c ) hybridizing to the first cDNA strand a second primer comprising a 3' random cDNA hybridizing sequence and a second 5' defined amplifyable sequence; d) extending the second primer with a DNA polymerase to form a second cDNA strand; and e) amplifying the second cDNA strand with a third primer comprising the first 5' defined amplifyable sequence. |
申请公布号 |
CA2416091(A1) |
申请公布日期 |
2002.01.24 |
申请号 |
CA20012416091 |
申请日期 |
2001.07.17 |
申请人 |
INCYTE GENOMICS, INC. |
发明人 |
DANIEL, STEVE;ARNOLD, LYLE;BJELDANES, ERIK |
分类号 |
C12N15/09;C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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