| 摘要 |
PURPOSE: A gene encoding cysteine proteinase derived from Paragonimus westermani Brown and use of cysteine proteinase are provided, thereby reducing the cross-reaction with other bacteria, and increasing sensitivity of diagnosis when the cysteine proteinase is used for diagnosis of infection of Paragonimus westermani Brown, so that accuracy of the diagnosis can be improved. CONSTITUTION: The gene encoding cysteine proteinase derived from Paragonimus westermani Brown has the nucleotide sequence set forth in SEQ ID NO:1, wherein the cysteine proteinase gene is isolated and sequenced by the steps of: preparing primers, primer 1 and primer 2 using beta-cyanoethyl phosphoramidite in which the primer 1 has the nucleotide sequence of 5-ACA GAA TTC CAR GGI CAR TGY GGI TCI TGY TGG-3, and the primer 2 has the nucleotide sequence of 5-TTA AAG CTT CCA IGA RTT YTT IAC RAT CCA RTA-3, wherein I is inosine, R is A or C, and Y is T or C; reacting cDNA derived from mRNA of Paragonimus westermani Brown with the primers to isolate DNA fragments associated with the cysteine proteinase; and sequencing the DNA fragments. A composition for diagnosis of infection of Paragonimus westermani Brown comprises the cysteine proteinase as an effective component.
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