ACTIVATION OF GENE EXPRESSION IN CLONED EUKARYOTIC GENOMIC DNA AND METHODS OF USE THEREOF

摘要

The present invention provides methods of modulating the expression of eukaryotic genes in eukaryotic cells. The methods employ homologous recombination to insert heterologous promoters and transcriptional control sequences into independent origin based cloning vectors (IOBCVs) that comprise eukaryotic genes. The resulting modified IOBCVs can then be inserted into eukaryotic cells to express the eukaryotic genes. Included in the invention are methods of using the vectors to modulate the expression of the eukaryotic genes in cells and in animals that comprise the cells. Also included in the invention are methods of preparing IOBCV expression contigs and genomic DNA expression libraries.