发明名称 |
GENERATION AND SCREENING OF A DYNAMIC COMBINATORIAL LIBRARY |
摘要 |
The present invention concerns a method for selectively establishing a dynamic combinatorial library of ligands for a target which binds at least two functionalities, which method comprises the following steps: selecting a plurality of functionalities which upon combination with each other are capable of forming an entity which may bind to the at least two functionalities in the target; selecting at least one spacer group to be located between the at least two functionalities, which spacer group is of an appropriate size and/or flexibility to allow the functionalities to fit into the binding sites on the target and allowing a reversible bond formation and cleavage; creating discrete ligands by linking at least two identical or different functionalities by at least one spacer group or; linking the functionalities to fragments of the spacer group which contain functions allowing the said reversible bond formation under formation of the spacer group and cleavage of it; mixing together a plurality of different discrete ligands and/or different functionalities; subjecting the mixture to conditions allowing a reversible bond formation and cleavage; adding the target to the mixture; identifying the functionality combinations which are most appropriate for the formation of a complex between the target and the active molecule. In a further embodiment of the invention, the target is added when the discrete ligands are mixed together, in order to be present when the scrambling takes place.
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申请公布号 |
WO0164605(A3) |
申请公布日期 |
2001.12.27 |
申请号 |
WO2001EP02310 |
申请日期 |
2001.03.01 |
申请人 |
THERASCOPE AG;LEHN, JEAN-MARIE;RAMSTROEM, OLOF |
发明人 |
LEHN, JEAN-MARIE;RAMSTROEM, OLOF |
分类号 |
C07H15/04;A61K47/48;C07B61/00;C07H15/203;C07H15/207;C07H15/22;G01N33/53;G01N33/531;(IPC1-7):C07B61/00 |
主分类号 |
C07H15/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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