Immunosorbant assay using branched bis-biotin/avidin/multiple label complex as a detection reagent

摘要

The present invention relates to a branched bis-biotin/avidin/multiple label complex that is conjugated to a member of a specific binding pair ("sbp member"). The complex and conjugate compositions of the invention find use in an assay for an analyte wherein there is employed a reagent system comprising an avidin reagent and a biotin reagent. The present invention comprises using as the biotin reagent the branched bis-biotin/avidin/multiple label complex as described above. Also disclosed are kits comprising the present bis-biotin/avidin/multiple label complex and methods of preparing a bis-biotin/avidin/multiple label complex conjugate of a member of a specific binding pair ("sbp member") for use in a specific binding assay.