发明名称 |
METHOD OF PRODUCING THY A<->STRAINS OF VIBRIO CHOLERAE, SUCH STRAINS AND THEIR USE |
摘要 |
A method of producing a thy A<->strain of vibrio cholerae comprising the step of site-directed mutagenesis in the V. cholerae chromosome at the locus of the thy A gene SEQ ID NO: 1 of FIG. 1, is described. Particularly, a DELTA thy A strain of Vibrio cholerae lacking the functionality of the thy A is disclosed. This strain may comprise one or several episomal autonomously replicating DNA elements, such as plasmids, having an optionally foreign, e.g. E. coli, functional thy A gene that enables the strain to grow in the absence of thymine in the growth medium, and optionally having a structural gene encoding a homologous or heterologous protein. Further, proteins encoded by a structural thy A gene and the 5'-flanking region are described as SEQ ID NO: 4 of FIG. 4 and SEQ ID NO: 5 of FIG. 5, respectively. Additionally, a vaccine comprising a Vibrio cholerae DELTA thy A strain of the invention or a thy A<->strain of Vibrio cholerae produced by the method of the invention is disclosed.
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申请公布号 |
WO9961634(A9) |
申请公布日期 |
2001.12.13 |
申请号 |
WO1999EP03509 |
申请日期 |
1999.05.21 |
申请人 |
ACTIVE BIOTECH AB;CARLIN, NILS;LEBENS, MICHAEL, R. |
发明人 |
CARLIN, NILS;LEBENS, MICHAEL, R. |
分类号 |
C12N1/21;A61K39/00;A61K39/106;A61K39/108;C07K14/245;C07K14/28;C07K14/435;C12N9/10;C12N15/09;C12N15/31;C12N15/54;C12N15/74;C12R1/63;(IPC1-7):C12N15/74 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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