发明名称 |
METHOD FOR ELECTROPHORETICALLY SEPARATING MEMBRANE PROTEINS |
摘要 |
The one and two-dimensional separation of proteins extending through the membrane (membrane proteins) is a prerequisite for a complete analysis of th e proteome of a functionally intact cell organelle. A substantial drawback of prior art methods for separating is that their applicability is limited to peripheral water-soluble proteins. The aim of the novel method is to electrophoretically separate membrane proteins in one or two dimensions. To this end, membranes from cell organelles, whose membrane protein stock shoul d be separated, are placed on planar supports whose surface was modified using suitable methods in such a manner that the membrane proteins maintain their ability to laterally diffuse in the membrane plane after being placed on the support. By subsequently applying electrical fields, the proteins can be electrophoretically displaced in the membrane plane and, as a result, can be separated in one or two dimensions according to their different charge characteristics.
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申请公布号 |
CA2411684(A1) |
申请公布日期 |
2001.12.13 |
申请号 |
CA20012411684 |
申请日期 |
2001.06.01 |
申请人 |
NIMBUS BIOTECHNOLOGIE GMBH |
发明人 |
SACKMANN, ERICH;BAYERL, THOMAS |
分类号 |
C07K1/26;C07K14/705;(IPC1-7):C07K1/26 |
主分类号 |
C07K1/26 |
代理机构 |
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