发明名称 |
Use of dimly fluorescing nucleic acid dyes in the identification of nucleated cells |
摘要 |
This invention presents improved methodology for identification of nucleated cells in flow cytometric analysis when immunofluorescent dyes are also used. Briefly, in the method nucleic acids are stained with a fluorescent dye which can then be used to identify the nucleated cells by measurement of fluorescence on a flow cytometer. The improvement presented by this invention is the use of a saturating (or near saturating) amount of a nucleic acid dye, or mixture of dyes, which gives low fluorescence at excitation conditions, so as not to greatly interfere with the signals of the immunofluorescent dyes.
|
申请公布号 |
US6329158(B1) |
申请公布日期 |
2001.12.11 |
申请号 |
US19950528828 |
申请日期 |
1995.09.15 |
申请人 |
BECTON DICKINSON AND COMPANY |
发明人 |
HOFFMAN ROBERT A.;FREY THOMAS |
分类号 |
C12Q1/68;G01N21/64;G01N33/569;G01N33/58;(IPC1-7):G01N33/533;G01N33/53;G01N21/76 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|