| 摘要 |
Provided are methods to detect of modulators of the Receptor for Advanc ed Glycated Endproducts (RAGE). The invention comprises a method for detection of RAGE modulators comprising: adsorbing a RAGE ligand onto a solid surface; adding a compound of interest and a protein comprising RAGE or fragment thereof, to the preadsorbed ligand; adding an antibody which binds to RAGE or fragment thereof and a secondary antibody which binds to the anti-RAGE antibody; measuring the secondary antibody boun d to the anti-RAGE antibody; and comparing the amount of RAGE bound to the ligand in the presence of varying amounts of the compound of interest. In an embodiment, t he fragment of RAGE is sRAGE. In one aspect, the invention use of compounds detected by the method for treatment of AGE-related syndromes including complications associated wi th diabetes, kidney failure, lupus nephritis or inflammatory lupus nephritis, amyloidoses , Alzheimer's disease, cancer, inflammation, and erectile dysfunction.
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