alpha -ISOMALTOSYLTRANSFERASE, PROCESS FOR PRODUCING THE SAME AND USE THEREOF

摘要

alpha -Isomaltosyltransferase capable of forming a cyclic tetrasaccharide having a cyclo { - 6} - alpha -D-glucopyranosyl- (1 - 3) - alpha -D-glucopyranosyl- (1 - 6) - alpha -D-glucopyranosyl- ( 1 - 3) -a-D-glucopyranosyl- ( 1 - ) structure via a reaction involving alpha -isomaltosyl transfer starting from a saccharide having an alpha -1,6-glucosyl bond as the bonding manner at the non-reducing end and an alpha -1,4-glucosyl bond as the bonding manner at the part other than the reducing end and having a degree of glucose polymerization of at least 3 is provided. Also, a microorganism producing the above enzyme, a process for producing the above enzyme, the cyclic tetrasaccharide obtained by using the above enzyme or a carbohydrate containing the same, and use thereof are established.