| 摘要 |
Compounds termed "vitaletheine modulators" which include beta-alanyl-taurine and carbobenzoxy beta-alanyl-taurine are synthesized and added to culture media for in vitro culture of cells such as mammalian or plant cells. The compounds support cell vitality, and provide increased cellular life span, increased cellular bioproductivity, improved cellular function, and adaption of resistant cells to culturing. The compounds further delay senescence, optimize growth and maturation, and increase population doublings. Carbobenzoxy beta-alanyl-taurine is produced by coupling beta-alanine, which has it's terminal amine protected with a carbobenzoxy (CBZ) group, to N-hydroxysuccinimide to produce an active ester of beta-alanine, coupling two of the active esters to cystamine to produce a CBZ-protected beta-alethine having an internal disulfide bond, isolating and purifying the CBZ-protected beta-alethine, and reacting the CBZ-protected beta-alethine with iodine to oxidize the disulfide bond to obtain carbobenzoxy beta-alanyl-taurine. Beta-alanyl-taurine is produced by the same procedure except that after oxidizing with iodine, the carbobenzoxy beta-alanyl-taurine is irradiated with UV radiation to remove the CBZ-protecting group.
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