| 摘要 |
A method for determining amylase concentrations is provided, comprising the steps of: (a) incubating a part of a specimen having an unknown amylase concentration at a specified temperature in a water-miscible buffered starch solution containing starch at a concentration of between 20-200 mg/litre; (b) allowing the thus-obtained mixture to react for a specified time period; (c) adding a portion of an aqueous iodine solution to this mixture to obtain a first sample; (d) preparing a second sample by adding another part of the specimen to a mixture comprising a second portion of the iodine solution and a second portion of the starch solution; (e) measuring the optical density of both samples at a wavelength between 300-800 nm; and(f) calculating the amylase activity in the specimen using a calibration curve,wherein both the first and the second sample containequal concentrations of the specimen and the starch solution. The time needed for carrying out this method is less than 10 minutes, preferably less than 6 minutes. This method is suitable for monitoring the amylase concentration in industrial cleaning processes. |
