BIO-SAMPLE ANALYZING METHOD OF TRANSFORMED PLANT AND SYSTEM THEREOF

摘要

PURPOSE: A method for analyzing a bio-sample of a transformed plant and a system thereof are provided to simply and speedily detect the generation of GFP without any genes, substrate or auxiliary genes, thereby improving the study or development of gene generation of plant organisms or sorting of transformed seeds. CONSTITUTION: In a method for analyzing a bio-sample of a transformed plant, excited light is generated by using a light source(1), radiating light to a sample of a transformed plant exhibiting GFP(green fluorescent protein) fluorescence by an angle of 45° by passing light in the vicinity of 488nm which exciting the GFP by disposing blue band-pass filter(2) in front of the light source for passing only light of 470-490nm, transmitting light in the vicinity of 500-550nm with a zoom lens(5) by passing light in the vicinity of 509nm with a green band-pass filter(4) when the GFP in the sample becomes fluorescent, taking a photograph of an image by a CCD color video camera(6) attached with the zoom lens, and processing the image by a computer connected to the camera, thereby determining the transformation of the plant sample.