| 摘要 |
The invention relates to a laser microdissection device comprised of a microscope table ( 1 ), which supports a specimen ( 3 ) to be dissected, of an incident lighting device ( 7 ), a laser light source ( 5 ) and of an objective ( 10 ) for focussing the laser beam ( 18 ) of the laser light source ( 5 ) onto the specimen ( 3 ). According to the invention, the microscope table ( 1 ) is not moved during the dissecting process. A laser scanning device ( 9 ) is arranged in the incident lighting device ( 7 ), is comprised of two thick glass wedge plates ( 11 a , 11 b), which are tilted toward the optical axis ( 8 ) and can be rotated independently of one another around said optical axis ( 8 ). In addition to the beam deviation caused by the wedge angle of the wedge plates ( 11 a, 11 b), a beam offset of the laser beam ( 18 ) is produced by the thickness and the tilt of the wedge plates ( 11 a, 11 b). When both wedge plates ( 11 a, 11 b) are rotated, the beam deviation and the beam offset of the laser beam ( 18 ) are varied in such a manner that the laser beam ( 18 ) always passes through the middle of the objective pupil ( 19 ) and, at the same time, the beam is guided over the specimen ( 3 ) to be dissected by the beam deviation of the laser beam ( 18 ). |
