发明名称 |
Methods for generating phosphorylation site-specific immunological reagents |
摘要 |
The present invention provides methods for generating phosphorylation site-specific immunological reagents. More specifically, a phosphopeptide mimetic is incorporated into a polypeptide in place of a phosphorylated amino acid. The polypeptide is used as antigen by standard methods to generate either monoclonal or polyclonal antibodies which cross-react with the naturally phosphorylated polypeptide. The phosphopeptide mimetic preferably contains a non-hydrolyzable linkage from the appropriate carbon atom of the amino acid residue to a phosphate group. A preferred linkage is a CF2 group. Such a linkage is used to generate the phosphoserine mimetic F2Pab, which is incorporated into a polypeptide sequence derived from p53 to produce antibodies which recognize a specific phosphorylation state of p53. A CF2 group linkage is also used to produce the phosphothreonine mimetic F2Pmb, and to produce the phosphotyrosine mimetic, F2Pmp.
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申请公布号 |
US6309863(B1) |
申请公布日期 |
2001.10.30 |
申请号 |
US19990236415 |
申请日期 |
1999.01.25 |
申请人 |
BROOKHAVEN SCIENCE ASSOCIATES |
发明人 |
ANDERSON CARL W.;APPELLA ETTORE;SAKAGUCHI KAZUYASU |
分类号 |
A01K67/027;C07K1/113;C07K1/14;C07K16/00;C07K16/18;C07K16/32;C12P21/04;C12P21/08;(IPC1-7):C07K16/00 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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