发明名称 |
VERFAHREN ZUR HERSTELLUNG VON VIRUSIMPFSTOFF AUS ABGESCHWÄCHTEN VARICELLA ZOSTER VIREN |
摘要 |
<p>A process for culturing cells in monolayer which comprises : a. seeding a culture vessel with a cell stock in a minimal or rich medium and allowing the cells to plant for about 24-72 hours; b. removing the medium from the culture of step a. and replacing with fresh, rich medium, supplemented with an opitimized concentration of lipids and optionally also including a serum supplement; c. growing the culture of cells in rich medium supplemented with lipid; d . optionally replacing the medium of Step c. with fresh medium containing no lipid supplement, after about 24-72 hours growth.</p> |
申请公布号 |
AT206311(T) |
申请公布日期 |
2001.10.15 |
申请号 |
AT19930201521T |
申请日期 |
1993.05.27 |
申请人 |
MERCK & CO., INC. |
发明人 |
PROVOST, PHILLIP J.;KRAH, DAVID L.;FRIEDMAN, PAUL A. |
分类号 |
A61K39/00;A61K39/12;A61K39/25;A61P31/12;A61P31/22;C12N1/38;C12N5/00;C12N5/02;C12N5/071;C12N5/10;C12N7/04;C12N7/08;(IPC1-7):A61K39/25 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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