Cloning system used in the construction of homologous recombination vectors

摘要

The invention relates to a novel cloning system that consists of a vector and adapter system that substantially simplifies the construction of homologous recombination vectors used for the mutagenesis of genes in living eukaryotic cells. The invention further relates to a method for producing homologous recombination vectors as well as to the use of said cloning system for modifying the genome at defined loci in eukaryotic cells, especially in embryonic stem cells.