PROBE FOR CONSTRUCTING PROBE POLYMER, METHOD OF CONSTRUCTINGPROBE POLYMER AND UTILIZATION THEREOF

摘要

A method of assaying a target gene under isothermal conditions without using any enzyme. A double-stranded probe polymer is formed by hybridizing plural probe pairs, each consisting of a pair of probes having n (n >= 3) base sequence domains complementary to each other, in such a manner as to form alternate crossing. The base sequence at the branching point of the complementary base sequence domains is bonded to a G (guanine)-C (cytosine) bond to thereby construct a stable double-stranded probe polymer. A complex of a target gene with the probe polymer is formed by using a base sequence complementary to a part of the target gene as one of the complementary domai ns of the probe and thus the target gene is assayed.