PROCESS FOR PRODUCING ACTIVATED PROTEIN

摘要

The present invention relates to a process for activating a purified polypeptide, characterized by bringing the purified polypeptide having an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID NO: 1 into contact with copper ions, calcium ions or magnesium ions, or a substance providing copper ions, a substance providing calcium ions, or a substance providing magnesium ions. The methods for activation or manufacturing of the present invention enable the inexpensive mass production of active MPIF-1 DELTA 23 which protects myeloid stem cells from chemotherapeutic drugs and permits rapid recovery from leukopenia, which is a limiting factor in the administration of anti-tumor agents.