Pullulanase expression constructs containing alpha-amylase promoter and leader sequences

摘要

Disclosed herein are DNA expression constructs containing an alpha-amylase promoter sequence derived from Bacillus stearothermophilus, an alpha-amylase leader sequence derived from Bacillus stearothermophilus, and a DNA sequence encoding a pullulanase derived from Bacillus naganoensis. Microbial hosts transformed to contain the expression constructs secret function pullulanases. Also disclosed is a process for making recombinant pullulanases utilizing the expression constructs and a recombinant pullulanase which can be produced in Bacillus subtilis.