发明名称 |
Generation and amplification of nucleic acids from ribonucleic acids |
摘要 |
Novel compositions and methods useful for the generation of nucleic acids from a ribonucleic acid template and further nucleic acid replication are disclosed. It is shown that the generation and amplification of nucleic acids by methods that utilize two or more different polymerases, such as reverse transcriptase-polymerase chain reaction (RT-PCR), are dramatically more sensitive and efficient in the presence of a homopolymeric nucleic acid. Homopolymeric nucleic acids have been found to reduce or negate the inhibitory effect reverse transcriptases have on DNA polymerase activity. It is demonstrated that this inhibition-relieving effect of homopolymeric nucleic acids is general in nature; independent of the chemical species of homopolymer used, or the chemical composition of the polymerization reaction mixture.
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申请公布号 |
US6300069(B1) |
申请公布日期 |
2001.10.09 |
申请号 |
US19990304452 |
申请日期 |
1999.05.03 |
申请人 |
QIAGEN GMBH |
发明人 |
MISSEL ANDREAS;LOEFFERT DIRK;KANG JIE;KORFHAGE CHRISTIAN |
分类号 |
C12N15/09;C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;C12N9/00;C07H21/02;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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